DOI: 10.36871/vet.zoo.bio.202107004
UDC 579.861.2

Authors

E. A. Sadovaya
Bachelor of Biology of Veterinary-Biological Faculty of Moscow State Academy of Veterinary Medicine and Biotechnology – MVA by K. I. Skryabin, Moscow, Russian Federation
O. B. Litvinov
Doctor of Veterinary Sciences, Professor, Department of Immunology and Biotechnology, Moscow State Academy of Veterinarian Medicine and Biotechnology – MVA by K. I. Skryabin, Moscow, Russian Federation
A. I. Laishevtsev
Candidate of Biological Sciences, Head of Laboratory of Diagnostics and Control of Antibiotic Resistance of Mostly Clinical Important Agents of Infectious Animal Diseases of Federal Scientific Center – All-Russian Scientific Research Institute of Experimental Veterinary Medicine named after K. I. Skryabin and Ya. R. Kovalenko of the Russian Academy of Sciences, Moscow, Russian Federation

Abstract

The article presents the results of determination of species variety of staphylococcus agents taken from poultry by the last few years.
The laboratory diagnostic research was done in the Laboratory of diagnostics and control of antibiotic resistance of mostly clinical important agents of infectious animal diseases of Federal Scientific Center – All-Russian Scientific Research Institute of Experimental Veterinary Medicine named after K.I.Skryabin and Ya. R. Kovalenko of the Russian Academy of Sciences. Pathologic material from broiler chickens and turkey of different age groups (from 1 to 70 days old) was analyzed by the scheme of laboratory diagnostic research used in the Laboratory.
The bacterial inoculation from different organs to blood agar was done, then we chose pure cultures by inoculating on sectors, after this we washed the culture to test-tubes and sent them for species determination by using the method of MALDI-TOF MS.
Selected staphylococci cultures were analyzed for antibiotic resistance.
The pathogenicity of the selected Staphylococcus epidermidis culture was by doing the bioassay on laboratory mice.
Necropsy of mice, in smears-prints and inoculations of cultures of typical growth for staphylococci were not found, which suggests that the culture of Staphylococcus epidermidis isolated from poultry is non-pathogenic.

Keywords

staphylococcosis, staphylococcus, agricultural poultry, MALDI-TOF MS, bioassay, pathogenicity, antibiotic resistance.