DOI: 10.36871/vet.zoo.bio.202107004
UDC 579.861.2
Authors
E. A. Sadovaya
Bachelor of Biology of Veterinary-Biological Faculty
of Moscow State Academy of Veterinary Medicine
and Biotechnology – MVA by K. I. Skryabin,
Moscow, Russian Federation
O. B. Litvinov
Doctor of Veterinary Sciences, Professor, Department
of Immunology and Biotechnology, Moscow State
Academy of Veterinarian Medicine and
Biotechnology – MVA by K. I. Skryabin,
Moscow, Russian Federation
A. I. Laishevtsev
Candidate of Biological Sciences, Head of Laboratory of Diagnostics
and Control of Antibiotic Resistance of Mostly Clinical Important
Agents of Infectious Animal Diseases of Federal Scientific
Center – All-Russian Scientific Research Institute
of Experimental Veterinary Medicine named
after K. I. Skryabin and Ya. R. Kovalenko
of the Russian Academy of Sciences,
Moscow, Russian Federation
Abstract
The article presents the results of determination of species variety of staphylococcus agents taken
from poultry by the last few years.
The laboratory diagnostic research was done in the Laboratory of diagnostics and control of antibiotic
resistance of mostly clinical important agents of infectious animal diseases of Federal Scientific
Center – All-Russian Scientific Research Institute of Experimental Veterinary Medicine named
after K.I.Skryabin and Ya. R. Kovalenko of the Russian Academy of Sciences. Pathologic material
from broiler chickens and turkey of different age groups (from 1 to 70 days old) was analyzed by the
scheme of laboratory diagnostic research used in the Laboratory.
The bacterial inoculation from different organs to blood agar was done, then we chose pure cultures
by inoculating on sectors, after this we washed the culture to test-tubes and sent them for
species determination by using the method of MALDI-TOF MS.
Selected staphylococci cultures were analyzed for antibiotic resistance.
The pathogenicity of the selected Staphylococcus epidermidis culture was by doing the bioassay
on laboratory mice.
Necropsy of mice, in smears-prints and inoculations of cultures of typical growth for staphylococci
were not found, which suggests that the culture of Staphylococcus epidermidis isolated from
poultry is non-pathogenic.
Keywords
staphylococcosis, staphylococcus, agricultural poultry, MALDI-TOF MS, bioassay, pathogenicity, antibiotic resistance.