Histochemical determination of flavonoid localization in epidermal tissues of medicinal plants Dioscorea caucasica Lipsky, Taxus canadensis Marsh, Ginkgo biloba, Helianthus annuus L.

UDC 574.577
DOI: 10.36871/vet.zoo.bio.202202009

Authors

Svetlana M. Zaytseva,
Elena A. Kalashnikova,
Rima N. Kirakosyan,
Irina A. Berkovskaya,
Airita S. Belevtsova,
Anastasia A. Shcherbakova,
State Agrarian University – Moscow State Agricultural Academy named after K. A. Timiryazev, Moscow, Russia

Abstract

Intracellular and tissue localization of soluble phenolic compounds of the flavonoid series was studied on the example of flavans and proanthocyanidins in the integumentary tissues of medicinal plants. The work used both well-known medicinal plants rich in various phenolic compounds (Dioscorea caucasica Lipsky, Taxus canadensis Marsh, Ginkgo biloba) and traditional agricultural crops that occupy leading positions in feed production (Helianthus annuus L.) On the basis of specific histochemical reactions revealed that flavans were major components and were ubiquitously present in the integumentary tissues of the studied plants. Specific staining for the amount of soluble phenolic compounds coincided with histochemical reactions with vanillin reagent. Polyphenols in these plants were localized in epidermal tissues, in exogenous excretory structures, as well as in glandular hairs. In the cell walls, intercellular cells and vacuoles of epiblasts, they were present in the form of amorphous matter and granular inclusions.

Keywords

phenolic compounds, flavans, localization, dioscorea, yew, double-lobed ginkgo, sunflower