UDC 619: 616.9: 636.088: 636.4
DOI: 10.36871/vet.zoo.bio.202303004
Authors
Regan Reddy Gade,
Sergey V. Fedotov,
Moscow State Academy of Veterinary Medicine and Biotechnology – MVA by K. I. Skryabin”, Moscow, Russia
Pavel G. Simonov,
Altai State Agrarian University, Barnaul, Russia
Abstract
The polyetiology of postpartum mastitis and endometritis makes it difficult to develop and
use specific drugs. Therefore, there is a need for a more detailed study of the microbiological
composition of the udder microflora, which will make it possible to correctly and appropriately
select antimicrobial drugs.
The main properties of microorganisms isolated from postpartum mastitis and endometritis in
cows were studied. Of 166 samples from the exudate of the uterus and udder of cows, 32 (19,28 %)
cultures of Escherichia coli (E. coli) were isolated, while in 14 cultures (43,75 %) hemolytic
activity was confirmed, and 17 cultures (53,12 %) were highly pathogenic for white mice. In 35
samples (21,08 % of the total number of cultures), bacteria of the genus Klebsiella were isolated,
active hemolysis was observed in 12 (34,28 %) samples, 16 (45,71 %) cultures were pathogenic
for white mice. Bacteria of the genus Proteus were isolated in 24 samples (14,46 %) of the total
number of cultures, hemolytic activity was detected in 11 (45,83 %) cultures, pathogenicity for
laboratory animals was determined in 9 cultures (37,50 %).
The analysis of isolated microorganisms from the contents of the uterus and udder from cows
showed that the species composition of the microflora in postpartum mastitis and endometritis is
practically the same, which gives us the opportunity to assume that pathogenic microorganisms
can be transferred from the focus of inflammation in the mammary gland by lymphogenous or
hematogenous way to the uterus, and back.
With an increase in the number of postpartum mastitis and endometritis in cows in large
livestock complexes with an intensive housing system, it is advisable to use modern PCR
diagnostics. Real-time PCR has significant advantages over traditional methods, as well as
higher sensitivity and specificity.
Keywords
cows, postpartum mastitis, postpartum endometritis, microorganisms, PCR
