УДК 612.32:579:636.3+612.33:636.3
DOI: 10.36871/vet.zoo.bio.202306013
Authors
Vladimir A. Devyatkin,
Federal Research Center for Animal Husbandry named after Academy Member L. K. Ernst,
Dubrovitsy, Russia
Abstract
A method of optimizing digestive processes in the pre-ventricles of sheep, improving rumen and
intestinal microbiocenoses, increasing natural resistance through the use of a prebiotic supplement
is considered. Feeding homogenate (group 1) and supernatant (group 2) of the Aspergillus
oryzae culture fluid to Romanov breed boulders contributed to an increase in the microbial mass
of the rumen in the first group by 72,9 %, in the second by 76,0 %, more intensive hydrolysis of
carbohydrates due to an increase in the level of LVH by 37,9 (p<0,01) and 47,4 % (p<0,01), acidic
metabolites – by 0,46 (p<0.01) and 0,44 (p<0,05) units, an increase in the concentration of ammonia
by 36,1 % and 37,7 % compared with the initial values. Biochemical parameters of protein, carbohydrate
and mineral metabolism were within the physiological norm.
Cellular immunity in animals of the experimental groups turned out to be more stable:
percent lysis was lower by 14,91 and 16,8 %, bacterial activity was higher than control values by
31,2 and 26,2 %, phagocytic by 1,8 and 2,2 % (p<0,05). The content of lacto- and bifidobacteria in
the large intestine was at the level of 5,49–5,18 and 6,65–7,38 lg CFU/g (p>0,05) versus 6,88 and
7,46 lg CFU/g in the control, a decrease of 3,3–2,57 lg CFU/g (p<0.01) of lactose-positive E. coli,
inhibition of mold growth by 17,9–8,3 % in the experimental groups indicates an increased
resistance of the animals of the experimental groups to infectious and inflammatory processes,
an increase in natural resistance and the positive role of the studied factors in the correction of
intestinal microbiocenosis of sheep.
Keywords
sheep, culture fluid of the homogenate and supernatant of the fungus Aspergillus oryzae F-155, rumen microflora, nonspecific resistance
